High accuracy Progesterone ELISA TEST KIT

INTENDED USE

The Progesterone assay is an enzyme-linked immunosorbent assay (ELISA) for the in vitro quantitative determination of progesterone concentration in human serum. For professional use only.

Test principle

Competition principle.

Total duration of assay: 80 minutes.

• Sample, Progesterone derivant coated microwells and enzyme labeled Progesterone antibodies are combined.

• During the incubation, Progesterone derivant coated on microwells and Progesterone present in the sample compete for binding to the enzyme labeled antibodies.

• After washing, a complex is generated between the solid phase and enzyme-linked antibodies by immunological reactions.

• Substrate solution is then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance.

• The color intensity is inversely proportional to the amount of Progesterone in the sample.

Specimen collection and preparation

• Human serum is recommended for this assay.

• Cap and store the samples at 18-25 ℃ for no more than 8 hours. Stable for 7 days at 2-8℃, and 1 month at -20℃. Freeze only once.

• Do not use heat-inactivated samples.

• Sediments and suspended solids in samples may interfere with the test result which should be removed by centrifugation. Ensure that complete clot formation in serum samples has taken place prior to centrifugation.

• Avoid grossly hemolytic, lipemic or turbid samples.

Storage and stability

• Store at 2-8℃.

• Seal and return unused reagents to 2-8℃, under which conditions the stability will be retained for 2 months, or until the labeled expiry date, whichever is earlier.

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